Formaldehyde scavengers function as novel antigen retrieval agents.

ABSTRACT: Antigen retrieval agents improve the detection of formaldehyde-fixed proteins, but how they work is not well understood. We demonstrate that formaldehyde scavenging represents a key characteristic associated with effective antigen retrieval; under controlled temperature and pH conditions, scavenging improves the typical antigen retrieval process through reversal of formaldehyde-protein adduct formation. This approach provides a rational framework for the identification and development of more effective antigen retrieval agents.

SUBMITTER: Vollert CT 

PROVIDER: S-EPMC4661444 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature