Variation of fibrinogen oligosaccharide structure in the acute phase response: Possible haemorrhagic implications.

ABSTRACT:

Background

Fibrinogen is an acute phase glycoprotein whose concentration increases in response to trauma. The newly synthesised protein is functionally enhanced and it is known that treatment with neuraminidase increases the rate of fibrin polymerisation. To explore this, we examined the differences between the oligosaccharide structures of quiescent and acute phase fibrinogen.

Methods

A series of plasma samples was obtained from two individuals suffering an acute phase response. Fibrinogen chains were examined directly by ESI mass spectrometry before and after digestion with N-glycosidase F and β1,4 galactosidase.

Results

The Bβ and γ chains of acute phase fibrinogen showed a mass decrease of 162 Da (Gal) in some 50% of the molecules, and the Bβ chain showed an addit

SUBMITTER: Brennan SO 

PROVIDER: S-EPMC4661561 | biostudies-literature | 2015 Jun

REPOSITORIES: biostudies-literature