Conservation and Role of Electrostatics in Thymidylate Synthase.

ABSTRACT: Conservation of function across families of orthologous enzymes is generally accompanied by conservation of their active site electrostatic potentials. To study the electrostatic conservation in the highly conserved essential enzyme, thymidylate synthase (TS), we conducted a systematic species-based comparison of the electrostatic potential in the vicinity of its active site. Whereas the electrostatics of the active site of TS are generally well conserved, the TSs from minimal organisms do not conform to the overall trend. Since the genomes of minimal organisms have a high thymidine content compared to other organisms, the observation of non-conserved electrostatics was surprising. Analysis of the symbiotic relationship between minimal organisms and their hosts, and the genetic completenes

SUBMITTER: Garg D 

PROVIDER: S-EPMC4661567 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature