A Novel High-Throughput Approach to Measure Hydroxyl Radicals Induced by Airborne Particulate Matter.

ABSTRACT: Oxidative stress is one of the key mechanisms linking ambient particulate matter (PM) exposure with various adverse health effects. The oxidative potential of PM has been used to characterize the ability of PM induced oxidative stress. Hydroxyl radical (•OH) is the most destructive radical produced by PM. However, there is currently no high-throughput approach which can rapidly measure PM-induced •OH for a large number of samples with an automated system. This study evaluated four existing molecular probes (disodium terephthalate, 3'-p-(aminophenyl)fluorescein, coumarin-3-carboxylic acid, and sodium benzoate) for their applicability to measure •OH induced by PM in a high-throughput cell-free system using fluorescence techniques, based on both our experiments and on an assessment of the phy

SUBMITTER: Son Y 

PROVIDER: S-EPMC4661607 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature