Construction of a high-density mutant library in soybean and development of a mutant retrieval method using amplicon sequencing.

ABSTRACT:

Background

Functions of most genes predicted in the soybean genome have not been clarified. A mutant library with a high mutation density would be helpful for functional studies and for identification of novel alleles useful for breeding. Development of cost-effective and high-throughput protocols using next generation sequencing (NGS) technologies is expected to simplify the retrieval of mutants with mutations in genes of interest.

Results

To increase the mutation density, seeds of the Japanese elite soybean cultivar Enrei were treated with the chemical mutagen ethyl methanesulfonate (EMS); M2 seeds produced by M1 plants were treated with EMS once again. The resultant library, which consisted of DNA and seeds from 1536 plants, revealed large morphological and physiological

SUBMITTER: Tsuda M 

PROVIDER: S-EPMC4662035 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature