Cross-species Transcriptomic Comparison of In Vitro and In Vivo Mammalian Neural Cells.

ABSTRACT: The mammalian brain is characterized by distinct classes of cells that differ in morphology, structure, signaling, and function. Dysregulation of gene expression in these cell populations leads to various neurological disorders. Neural cells often need to be acutely purified from animal brains for research, which requires complicated procedure and specific expertise. Primary culture of these cells in vitro is a viable alternative, but the differences in gene expression of cells grown in vitro and in vivo remain unclear. Here, we cultured three major neural cell classes of rat brain (ie, neurons, astrocytes, and oligodendrocyte precursor cells [OPCs]) obtained from commercial sources. We measured transcript abundance of these cell types by RNA sequencing (RNA-seq) and compared with their co

SUBMITTER: LoVerso PR 

PROVIDER: S-EPMC4662426 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature