Cross-correlated relaxation measurements under adiabatic sweeps: determination of local order in proteins.

ABSTRACT: Adiabatically swept pulses were originally designed for the purpose of broadband spin inversion. Later, unexpected advantages of their utilization were also found in other applications, such as refocusing to excite spin echoes, studies of chemical exchange or fragment-based drug design. Here, we present new experiments to characterize fast (ps-ns) protein dynamics, which benefit from little-known properties of adiabatic pulses. We developed a strategy for measuring cross-correlated cross-relaxation (CCCR) rates during adiabatic pulses. This experiment provides a linear combination of longitudinal and transverse CCCR rates, which is offset-independent across a typical amide (15)N spectrum. The pulse sequence can be recast to provide accurate transverse CCCR rates weighted by the populations

SUBMITTER: Kaderavek P 

PROVIDER: S-EPMC4662729 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature