Molecular Detection and Identification of Zoonotic Microsporidia Spore in Fecal Samples of Some Animals with Close-Contact to Human.

ABSTRACT:

Background

Microsporidia species are obligatory intracellular agents that can infect all major animal groups including mammals, birds, fishes and insects. Whereas worldwide human infection reports are increasing, the cognition of sources of infection particularly zoonotic transmission could be helpful. We aimed to detect zoonotic microsporidia spore in fecal samples from some animals with close - contact to human.

Methods

Overall, 142 fecal samples were collected from animals with closed-contact to human, during 2012-2013. Trichrome - blue staining were performed and DNA was then extracted from samples, identified positive, microscopically. Nested PCR was also carried out with primers targeting SSU rRNA gene and PCR products were sequenced.

Results

From 142 stool samp

SUBMITTER: Askari Z 

PROVIDER: S-EPMC4662738 | biostudies-literature | 2015 Jul-Sep

REPOSITORIES: biostudies-literature