Characterization of Specific RAPD Markers of Virulence in Trichomonas vaginalis Isolates.

ABSTRACT:

Background

As for human trichomoniasis the host-parasite relationship is very complex, and the broad ranges of clinical symptoms are unlikely be attributable to a single pathogenic mechanism. Specific Random Amplified Polymorphic DNA (RAPD) markers of 490 bp, 720 bp and 460 bp using the primers Tv-5, OPA-6 and OPA-11, respectively, were reported. This was the first description of possible genetic virulence markers of the infection by T. vaginalis. The aim of this study was to characterize the specific RAPD markers in order to elucidate their importance on virulence of this illness.

Methods

The selected specific RAPD fragments were cloned and sequenced. The obtained sequences were compared by the BLAST algorithm.

Results

The nucleotide sequence of the Tv-5490 RAPD mark

SUBMITTER: Fraga J 

PROVIDER: S-EPMC4662745 | biostudies-literature | 2015 Jul-Sep

REPOSITORIES: biostudies-literature