Pfcrt Gene in Plasmodium falciparum Field Isolates from Muzaffargarh, Pakistan.

ABSTRACT:

Background

The aim of the study was to identify the prevalence of different species of Plasmodium and haplotypes of pfcrt in Plasmodium falciparum from the selected area.

Methods

Overall, 10,372 blood films of suspected malarial patients were examined microscopically from rural health center Sinawan, district Muzaffargarh, Pakistan from November 2008 to November 2010. P. falciparum positive samples (both whole blood and FTA blood spotted cards) were used for DNA extraction. Nested PCR was used to amplify the pfcrt (codon 72-76) gene fragment. Sequencing was carried out to find the haplotypes in the amplified fragment of pfcrt gene.

Result

Over all slide positivity rate (SPR), P. vivax and P. falciparum positivity rate was 21.40 %, 19.37 % and 2.03% respectively. FTA b

SUBMITTER: Sahar S 

PROVIDER: S-EPMC4662792 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature