Induction of targeted, heritable mutations in barley and Brassica oleracea using RNA-guided Cas9 nuclease.

ABSTRACT:

Background

The RNA-guided Cas9 system represents a flexible approach for genome editing in plants. This method can create specific mutations that knock-out or alter target gene function. It provides a valuable tool for plant research and offers opportunities for crop improvement.

Results

We investigate the use and target specificity requirements of RNA-guided Cas9 genome editing in barley (Hordeum vulgare) and Brassica oleracea by targeting multicopy genes. In barley, we target two copies of HvPM19 and observe Cas9-induced mutations in the first generation of 23 % and 10 % of the lines, respectively. In B. oleracea, targeting of BolC.GA4.a leads to Cas9-induced mutations in 10 % of first generation plants screened. In addition, a phenotypic screen identifies T0 plants with t

SUBMITTER: Lawrenson T 

PROVIDER: S-EPMC4663725 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature