Protocol Improvements for Low Concentration DNA-Based Bioaerosol Sampling and Analysis.

ABSTRACT:

Introduction

As bioaerosol research attracts increasing attention, there is a need for additional efforts that focus on method development to deal with different environmental samples. Bioaerosol environmental samples typically have very low biomass concentrations in the air, which often leaves researchers with limited options in choosing the downstream analysis steps, especially when culture-independent methods are intended.

Objectives

This study investigates the impacts of three important factors that can influence the performance of culture-independent DNA-based analysis in dealing with bioaerosol environmental samples engaged in this study. The factors are: 1) enhanced high temperature sonication during DNA extraction; 2) effect of sampling duration on DNA recoverability

SUBMITTER: Luhung I 

PROVIDER: S-EPMC4664469 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature