Production of the Main Celiac Disease Autoantigen by Transient Expression in Nicotiana benthamiana.

ABSTRACT: Celiac Disease (CD) is a gluten sensitive enteropathy that remains widely undiagnosed and implementation of massive screening tests is needed to reduce the long term complications associated to untreated CD. The main CD autoantigen, human tissue transglutaminase (TG2), is a challenge for the different expression systems available since its cross-linking activity affects cellular processes. Plant-based transient expression systems can be an alternative for the production of this protein. In this work, a transient expression system for the production of human TG2 in Nicotiana benthamiana leaves was optimized and reactivity of plant-produced TG2 in CD screening test was evaluated. First, a subcellular targeting strategy was tested. Cytosolic, secretory, endoplasmic reticulum (C-terminal SEKDE

SUBMITTER: Marin Viegas VS 

PROVIDER: S-EPMC4664624 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature