The effect of Vdr gene ablation on global gene expression in the mouse placenta.

ABSTRACT: The effects of vitamin D are mediated through the vitamin D receptor (VDR), a predominantly nuclear receptor, expressed in numerous tissues including the placenta. VDR and the retinoid X receptor (RXR) form a dimer complex which binds to genomic vitamin D responsive elements located primarily in promoter regions and recruit cell-specific transcription factor complexes which regulate the expression of numerous genes. To investigate the role of VDR on regulating placental gene expression, mice heterozygous (+/-) for an ablated Vdr allele (C57Bl6 strain B6.129S4-VDRtm1Mbd/J, Jackson Laboratory) were mated to generate Vdr(+/+), Vdr(+/-) and Vdr (-/-) fetuses and placental samples were collected at day 18.5 of pregnancy. RNA was isolated from placental tissue with global gene expression measure

SUBMITTER: Buckberry S 

PROVIDER: S-EPMC4664714 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature