Peptidomic analysis reveals proteolytic activity of kefir microorganisms on bovine milk proteins.

ABSTRACT:

Scope

The microorganisms that make up kefir grains are well known for lactose fermentation, but the extent to which they hydrolyze and consume milk proteins remains poorly understood. Peptidomics technologies were used to examine the proteolytic activity of kefir grains on bovine milk proteins.

Methods and results

Gel electrophoresis revealed substantial digestion of milk proteins by kefir grains, with mass spectrometric analysis showing the release of 609 protein fragments and alteration of the abundance of >1500 peptides that derived from 27 milk proteins. Kefir contained 25 peptides identified from the literature as having biological activity, including those with antihypertensive, antimicrobial, immunomodulatory, opioid and anti-oxidative functions. 16S rRNA and shotgun

SUBMITTER: Dallas DC 

PROVIDER: S-EPMC4664887 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature