Ribosome profiling reveals an important role for translational control in circadian gene expression.

ABSTRACT: Physiological and behavioral circadian rhythms are driven by a conserved transcriptional/translational negative feedback loop in mammals. Although most core clock factors are transcription factors, post-transcriptional control introduces delays that are critical for circadian oscillations. Little work has been done on circadian regulation of translation, so to address this deficit we conducted ribosome profiling experiments in a human cell model for an autonomous clock. We found that most rhythmic gene expression occurs with little delay between transcription and translation, suggesting that the lag in the accumulation of some clock proteins relative to their mRNAs does not arise from regulated translation. Nevertheless, we found that translation occurs in a circadian fashion for many gene

SUBMITTER: Jang C 

PROVIDER: S-EPMC4665005 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature