Ribosome profiling reveals the rhythmic liver translatome and circadian clock regulation by upstream open reading frames.

ABSTRACT: Mammalian gene expression displays widespread circadian oscillations. Rhythmic transcription underlies the core clock mechanism, but it cannot explain numerous observations made at the level of protein rhythmicity. We have used ribosome profiling in mouse liver to measure the translation of mRNAs into protein around the clock and at high temporal and nucleotide resolution. We discovered, transcriptome-wide, extensive rhythms in ribosome occupancy and identified a core set of approximately 150 mRNAs subject to particularly robust daily changes in translation efficiency. Cycling proteins produced from nonoscillating transcripts revealed thus-far-unknown rhythmic regulation associated with specific pathways (notably in iron metabolism, through the rhythmic translation of transcripts containin

SUBMITTER: Janich P 

PROVIDER: S-EPMC4665006 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature