Glucocorticoid receptor and nuclear factor kappa-b affect three-dimensional chromatin organization.

ABSTRACT:

Background

The impact of signal-dependent transcription factors, such as glucocorticoid receptor and nuclear factor kappa-b, on the three-dimensional organization of chromatin remains a topic of discussion. The possible scenarios range from remodeling of higher order chromatin architecture by activated transcription factors to recruitment of activated transcription factors to pre-established long-range interactions.

Results

Using circular chromosome conformation capture coupled with next generation sequencing and high-resolution chromatin interaction analysis by paired-end tag sequencing of P300, we observed agonist-induced changes in long-range chromatin interactions, and uncovered interconnected enhancer-enhancer hubs spanning up to one megabase. The vast majority of activ

SUBMITTER: Kuznetsova T 

PROVIDER: S-EPMC4665721 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature