Expression, tissue localization and serodiagnostic potential of Taenia multiceps acidic ribosomal protein P2.

ABSTRACT:

Background

The larval stage of Taenia multiceps, also known as coenurus, is the causative agent of coenurosis, which results in severe health problems in sheep, goats, cattle and other animals that negatively impact on animal husbandry. There is no reliable method to identify coenurus infected goats in the early period of infection.

Methods

We identified a full-length cDNA that encodes acidic ribosomal protein P2 from the transcriptome of T. multiceps (TmP2). Following cloning, sequencing and structural analyses were performed using bioinformatics tools. Recombinant TmP2 (rTmP2) was prokaryotically expressed and then used to test immunoreactivity and immunogenicity in immunoblotting assays. The native proteins in adult stage and coenurus were located via immunofluorescence a

SUBMITTER: Huang X 

PROVIDER: S-EPMC4666187 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature