Detection of differentially methylated regions from whole-genome bisulfite sequencing data without replicates.

ABSTRACT: DNA methylation is an important epigenetic modification involved in many biological processes and diseases. Recent developments in whole genome bisulfite sequencing (WGBS) technology have enabled genome-wide measurements of DNA methylation at single base pair resolution. Many experiments have been conducted to compare DNA methylation profiles under different biological contexts, with the goal of identifying differentially methylated regions (DMRs). Due to the high cost of WGBS experiments, many studies are still conducted without biological replicates. Methods and tools available for analyzing such data are very limited.We develop a statistical method, DSS-single, for detecting DMRs from WGBS data without replicates. We characterize the count data using a rigorous model that accounts for t

SUBMITTER: Wu H 

PROVIDER: S-EPMC4666378 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature