In vivo imaging of axonal transport in murine motor and sensory neurons.

ABSTRACT:

Background

Axonal transport is essential for neuronal function and survival. Defects in axonal transport have been identified as an early pathological feature in several disorders of the nervous system. The visualisation and quantitative analysis of axonal transport in vivo in rodent models of neurological disease is therefore crucial to improve our understanding of disease pathogenesis and for the identification of novel therapeutics.

New method

Here, we describe a method for the in vivo imaging of axonal transport of signalling endosomes in the sciatic nerve of live, anaesthetised mice.

Results

This method allows the multiparametric, quantitative analysis of in vivo axonal transport in motor and sensory neurons of adult mice in control conditions and during disease

SUBMITTER: Gibbs KL 

PROVIDER: S-EPMC4666412 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature