The SUMOylation Pathway Restricts Gene Transduction by Adeno-Associated Viruses.

ABSTRACT: Adeno-associated viruses are members of the genus dependoviruses of the parvoviridae family. AAV vectors are considered promising vectors for gene therapy and genetic vaccination as they can be easily produced, are highly stable and non-pathogenic. Nevertheless, transduction of cells in vitro and in vivo by AAV in the absence of a helper virus is comparatively inefficient requiring high multiplicity of infection. Several bottlenecks for AAV transduction have previously been described, including release from endosomes, nuclear transport and conversion of the single stranded DNA into a double stranded molecule. We hypothesized that the bottlenecks in AAV transduction are, in part, due to the presence of host cell restriction factors acting directly or indirectly on the AAV-mediated gene tran

SUBMITTER: Holscher C 

PROVIDER: S-EPMC4666624 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature