Cas9 gRNA engineering for genome editing, activation and repression.

ABSTRACT: We demonstrate that by altering the length of Cas9-associated guide RNA (gRNA) we were able to control Cas9 nuclease activity and simultaneously perform genome editing and transcriptional regulation with a single Cas9 protein. We exploited these principles to engineer mammalian synthetic circuits with combined transcriptional regulation and kill functions governed by a single multifunctional Cas9 protein.

SUBMITTER: Kiani S 

PROVIDER: S-EPMC4666719 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature