Metagenomic sequencing of bile from gallstone patients to identify different microbial community patterns and novel biliary bacteria.

ABSTRACT: Despite the high worldwide prevalence of gallstone disease, the role of the biliary microbiota in gallstone pathogenesis remains obscure. Next-generation sequencing offers advantages for systematically understanding the human microbiota; however, there have been few such investigations of the biliary microbiome. Here, we performed whole-metagenome shotgun (WMS) sequencing and 16S rRNA sequencing on bile samples from 15 Chinese patients with gallstone disease. Microbial communities of most individuals were clustered into two types, according to the relative enrichment of different intestinal bacterial species. In the bile samples, oral cavity/respiratory tract inhabitants were more prevalent than intestinal inhabitants and existed in both community types. Unexpectedly, the two types were no

SUBMITTER: Shen H 

PROVIDER: S-EPMC4667190 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature