On the relevance of technical variation due to building pools in microarray experiments.

ABSTRACT:

Background

Pooled samples are frequently used in experiments measuring gene expression. In this method, RNA from different individuals sharing the same experimental conditions and explanatory variables is blended and their concentrations are jointly measured. As a matter of principle, individuals are represented in equal shares in each pool. However, some degree of disproportionality may arise from the limits of technical precision. As a consequence a special kind of technical error occurs, which can be modelled by a respective variance component. Previously published theory - allowing for variable pool sizes - has been applied to four microarray gene expression data sets from different species in order to assess the practical relevance of this type of technical error in terms of s

SUBMITTER: Rudolf H 

PROVIDER: S-EPMC4667463 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature