Identification of Nicotiana benthamiana microRNAs and their targets using high throughput sequencing and degradome analysis.

ABSTRACT:

Background

Nicotiana benthamiana is a widely used model plant species for research on plant-pathogen interactions as well as other areas of plant science. It can be easily transformed or agroinfiltrated, therefore it is commonly used in studies requiring protein localization, interaction, or plant-based systems for protein expression and purification. To discover and characterize the miRNAs and their cleaved target mRNAs in N. benthamiana, we sequenced small RNA transcriptomes and degradomes of two N. benthamiana accessions and validated them by Northern blots.

Results

We used a comprehensive molecular approach to detect and to experimentally validate N. benthamiana miRNAs and their target mRNAs from various tissues. We identified 40 conserved miRNA families and 18 novel mic

SUBMITTER: Baksa I 

PROVIDER: S-EPMC4667520 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature