Neuritic complexity of hippocampal neurons depends on WIP-mediated mTORC1 and Abl family kinases activities.

ABSTRACT:

Introduction

Neuronal morphogenesis is governed mainly by two interconnected processes, cytoskeletal reorganization, and signal transduction. The actin-binding molecule WIP (Wiskott-Aldrich syndrome protein [WASP]-interacting protein) was identified as a negative regulator of neuritogenesis. Although WIP controls activity of the actin-nucleation-promoting factor neural WASP (N-WASP) during neuritic differentiation, its implication in signal transduction remains unknown.

Methods

Using primary neurons from WIP-deficient and wild-type mice we did an immunofluorescence, morphometric, and biochemical analysis of the signaling modified by WIP deficiency.

Results

Here, we describe the WIP contribution to the regulation of neuritic elaboration and ramification through modific

SUBMITTER: Franco-Villanueva A 

PROVIDER: S-EPMC4667760 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature