Dietary salt regulates epithelial sodium channels in rat endothelial cells: adaptation of vasculature to salt.

ABSTRACT:

Background and purpose

The epithelial sodium channel (ENaC) is expressed in vascular endothelial cells and is a negative modulator of vasodilation. However, the role of endothelial ENaCs in salt-sensitive hypertension remains unclear. Here, we have investigated how endothelial ENaCs in Sprague-Dawley (SD) rats respond to high-salt (HS) challenge.

Experimental approach

BP and plasma aldosterone levels were measured. We used patch-clamp technique to record ENaC activity in split-open mesenteric arteries (MAs). Western blot and Griess assay were used to detect expression of α-ENaCs, eNOS and NO. Vasorelaxation in second-order MAs was measured with wire myograph assays.

Key results

Functional ENaCs were observed in endothelial cells and their activity was significantly de

SUBMITTER: Liu HB 

PROVIDER: S-EPMC4667865 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature