Deep Sequencing and Bioinformatic Analysis of Lesioned Sciatic Nerves after Crush Injury.

ABSTRACT: The peripheral nerve system has an intrinsic regenerative capacity in response to traumatic injury. To better understand the molecular events occurring after peripheral nerve injury, in the current study, a rat model of sciatic nerve crush injury was used. Injured nerves harvested at 0, 1, 4, 7, and 14 days post injury were subjected to deep RNA sequencing for examining global gene expression changes. According to the temporally differential expression patterns of a huge number of genes, 3 distinct phases were defined within the post-injury period of 14 days: the acute, sub-acute, and post-acute stages. Each stage showed its own characteristics of gene expression, which were associated with different categories of diseases and biological functions and canonical pathways. Ingenuity pathway

SUBMITTER: Yi S 

PROVIDER: S-EPMC4668002 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature