When cells divide: Label-free multimodal spectral imaging for exploratory molecular investigation of living cells during cytokinesis.

ABSTRACT: In vivo, molecular-level investigation of cytokinesis, the climax of the cell cycle, not only deepens our understanding of how life continues, but it will also open up new possibilities of diagnosis/prognosis of cancer cells. Although fluorescence-based methods have been widely employed to address this challenge, they require a fluorophore to be designed for a specific known biomolecule and introduced into the cell. Here, we present a label-free spectral imaging approach based on multivariate curve resolution analysis of Raman hyperspectral data that enables exploratory untargeted studies of mammalian cell cytokinesis. We derived intrinsic vibrational spectra and intracellular distributions of major biomolecular components (lipids and proteins) in dividing and nondividing human colon cance

SUBMITTER: Hsu JF 

PROVIDER: S-EPMC4668386 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature