Molecular cloning of Peking duck Toll-like receptor 3 (duTLR3) gene and its responses to reovirus infection.

ABSTRACT:

Background

Toll-like receptors (TLRs) play an important role in detecting pathogen-associated molecular patterns (PAMPs). Among the TLRs, TLR3 is involved in the recognition of double-stranded RNA. This study was designed to explore the relationship between duTLR3 and duck reovirus (DRV) infection.

Methods

In this study, we cloned and performed a molecular characterization of the complete sequence of Peking duck TLR3 (duTLR3). The expression level of duTLR3 was also determined, along with the relative levels of Mx and IFN-α mRNA after DRV infection.

Results

The duTLR3 gene is 2776-bp long and encodes an 895-amino-acid-long protein. Sequence analysis of the product revealed the complete transcript of Peking duck TLR3, including the 88-bp 5'UTR, the 2688-bp coding seque

SUBMITTER: Zhang M 

PROVIDER: S-EPMC4668636 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature