CpG island methylation profile in non-invasive oral rinse samples is predictive of oral and pharyngeal carcinoma.

ABSTRACT:

Background

There are currently no screening tests in routine use for oral and pharyngeal cancer beyond visual inspection and palpation, which are provided on an opportunistic basis, indicating a need for development of novel methods for early detection, particularly in high-risk populations. We sought to address this need through comprehensive interrogation of CpG island methylation in oral rinse samples.

Methods

We used the Infinium HumanMethylation450 BeadArray to interrogate DNA methylation in oral rinse samples collected from 154 patients with incident oral or pharyngeal carcinoma prior to treatment and 72 cancer-free control subjects. Subjects were randomly allocated to either a training or a testing set. For each subject, average methylation was calculated for each CpG

SUBMITTER: Langevin SM 

PROVIDER: S-EPMC4668652 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature