A single nucleotide change in mutY increases the emergence of antibiotic-resistant Campylobacter jejuni mutants.

ABSTRACT:

Objectives

Mutator strains play an important role in the emergence of antibiotic-resistant bacteria. Campylobacter jejuni is a leading cause of foodborne illnesses worldwide and is increasingly resistant to clinically important antibiotics. The objective of this study was to identify the genetic basis that contributes to a mutator phenotype in Campylobacter and determine the role of this phenotype in the development of antibiotic resistance.

Methods

A C. jejuni isolate (named CMT) showing a mutator phenotype was subjected to WGS analysis. Comparative genomics, site-specific reversion and mutation, and gene knockout were conducted to prove the mutator effect was caused by a single nucleotide change in the mutY gene of C. jejuni.

Results

The C. jejuni CMT isolate showed

SUBMITTER: Dai L 

PROVIDER: S-EPMC4668879 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature