Fitness of artemisinin-resistant Plasmodium falciparum in vitro.

ABSTRACT:

Objectives

Drug resistance confers a fitness advantage to parasites exposed to frequent drug pressure, yet these mutations also may incur a fitness cost. We assessed fitness advantages and costs of artemisinin resistance in Plasmodium falciparum in vitro to understand how drug resistance will spread and evolve in a competitive environment.

Methods

Genotyping of SNPs, drug susceptibility assays and copy number determination were used to assess the impact of artemisinin resistance on parasite fitness. An artemisinin-resistant clone (C9) selected in vitro from an isogenic parental clone (D6) was used to conduct competitive growth studies to assess fitness of artemisinin resistance. The resistant and susceptible clones were mixed or grown alone in the presence and absence of dru

SUBMITTER: Hott A 

PROVIDER: S-EPMC4668880 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature