A Quantitative System-Scale Characterization of the Metabolism of Clostridium acetobutylicum.

ABSTRACT:

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Engineering industrial microorganisms for ambitious applications, for example, the production of second-generation biofuels such as butanol, is impeded by a lack of knowledge of primary metabolism and its regulation. A quantitative system-scale analysis was applied to the biofuel-producing bacterium Clostridium acetobutylicum, a microorganism used for the industrial production of solvent. An improved genome-scale model, iCac967, was first developed based on thorough biochemical characterizations of 15 key metabolic enzymes and on extensive literature analysis to acquire accurate fluxomic data. In parallel, quantitative transcriptomic and proteomic analyses were performed to assess the number of mRNA molecules per cell for all genes under acidogenic, solventogenic, and al

SUBMITTER: Yoo M 

PROVIDER: S-EPMC4669385 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature