Optical super-resolution nanothermometry via stimulated emission depletion imaging of upconverting nanoparticles.

ABSTRACT: From engineering improved device performance to unraveling the breakdown of classical heat transfer laws, far-field optical temperature mapping with nanoscale spatial resolution would benefit diverse areas. However, these attributes are traditionally in opposition because conventional far-field optical temperature mapping techniques are inherently diffraction limited. Optical super-resolution imaging techniques revolutionized biological imaging, but such approaches have yet to be applied to thermometry. Here, we demonstrate a super-resolution nanothermometry technique based on highly doped upconverting nanoparticles (UCNPs) that enable stimulated emission depletion (STED) super-resolution imaging. We identify a ratiometric thermometry signal and maintain imaging resolution better than ~120

SUBMITTER: Ye Z 

PROVIDER: S-EPMC466949 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature