Time to Integrate to Nest Test Evaluation in a Mouse DSS-Colitis Model.

ABSTRACT: Severity assessment in laboratory animals is an important issue regarding the implementation of the 3R concept into biomedical research and pivotal in current EU regulations. In mouse models of inflammatory bowel disease severity assessment is usually undertaken by clinical scoring, especially by monitoring reduction of body weight. This requires daily observance and handling of each mouse, which is time consuming, stressful for the animal and necessitates an experienced observer. The time to integrate to nest test (TINT) is an easily applicable test detecting disturbed welfare by measuring the time interval mice need to integrate nesting material to an existing nest. Here, TINT was utilized to assess severity in a mouse DSS-colitis model. TINT results depended on the group size of mice ma

SUBMITTER: Hager C 

PROVIDER: S-EPMC4670219 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature