Innate and adaptive immunity to human beta cell lines: implications for beta cell therapy.

ABSTRACT: AIMS/HYPOTHESIS:Genetically engineered human beta cell lines provide a novel source of human beta cells to study metabolism, pharmacology and beta cell replacement therapy. Since the immune system is essentially involved in beta cell destruction in type 1 diabetes and after beta cell transplantation, we investigated the interaction of human beta cell lineswith the immune system to resolve their potential for immune intervention protocol studies. METHODS:Human pancreatic beta cell lines (EndoC-βH1 and ECi50) generated by targeted oncogenesis in fetal pancreas were assessed for viability after innate and adaptive immune challenges. Beta cell lines were pre-conditioned with T helper type 1 (Th1) cytokines or high glucose to mimic inflammatory and hyperglycaemia-stressed conditions. Beta cells

SUBMITTER: van der Torren CR 

PROVIDER: S-EPMC4670455 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature