Molecular, chemical and biological screening of soil actinomycete isolates in seeking bioactive peptide metabolites.

ABSTRACT:

Background and objective

Due to the evolution of multidrug-resistant strains, screening of natural resources, especially actinomycetes, for new therapeutic agents discovery has become the interests of researchers. In this study, molecular, chemical and biological screening of soil actinomycetes was carried out in order to search for peptide-producing actinomycetes.

Materials and methods

60 actinomycetes were isolated from soils of Iran. The isolates were subjected to molecular screening for detection NRPS (non-ribosomal peptide synthetases) gene. Phylogenic identification of NRPS containing isolates was performed. Chemical screening of the crude extracts was performed using chlorine o-dianisidine as peptide detector reagent and bioactivity of peptide producing strains was de

SUBMITTER: Hamedi J 

PROVIDER: S-EPMC4670464 | biostudies-literature | 2015 Feb

REPOSITORIES: biostudies-literature