A comprehensive Candida albicans PeptideAtlas build enables deep proteome coverage.

ABSTRACT: To provide new and expanded proteome documentation of the opportunistically pathogen Candida albicans, we have developed new protein extraction and analysis routines to provide a new, extended and enhanced version of the C. albicans PeptideAtlas. Two new datasets, resulting from experiments consisting of exhaustive subcellular fractionations and different growing conditions, plus two additional datasets from previous experiments on the surface and the secreted proteomes, have been incorporated to increase the coverage of the proteome. High resolution precursor mass spectrometry (MS) and ion trap tandem MS spectra were analyzed with three different search engines using a database containing allele-specific sequences. This approach, novel for a large-scale C. albicans proteomics project, was

SUBMITTER: Vialas V 

PROVIDER: S-EPMC4670622 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature