Towards the imaging of Weibel-Palade body biogenesis by serial block face-scanning electron microscopy.

ABSTRACT: Electron microscopy is used in biological research to study the ultrastructure at high resolution to obtain information on specific cellular processes. Serial block face-scanning electron microscopy is a relatively novel electron microscopy imaging technique that allows three-dimensional characterization of the ultrastructure in both tissues and cells by measuring volumes of thousands of cubic micrometres yet at nanometre-scale resolution. In the scanning electron microscope, repeatedly an image is acquired followed by the removal of a thin layer resin embedded biological material by either a microtome or a focused ion beam. In this way, each recorded image contains novel structural information which can be used for three-dimensional analysis. Here, we explore focused ion beam facilitated

SUBMITTER: Mourik MJ 

PROVIDER: S-EPMC4670698 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature