Systematic discovery of linear binding motifs targeting an ancient protein interaction surface on MAP kinases.

ABSTRACT: Mitogen-activated protein kinases (MAPK) are broadly used regulators of cellular signaling. However, how these enzymes can be involved in such a broad spectrum of physiological functions is not understood. Systematic discovery of MAPK networks both experimentally and in silico has been hindered because MAPKs bind to other proteins with low affinity and mostly in less-characterized disordered regions. We used a structurally consistent model on kinase-docking motif interactions to facilitate the discovery of short functional sites in the structurally flexible and functionally under-explored part of the human proteome and applied experimental tools specifically tailored to detect low-affinity protein-protein interactions for their validation in vitro and in cell-based assays. The combined com

SUBMITTER: Zeke A 

PROVIDER: S-EPMC4670726 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature