In Vitro Effects of Bisphenol A β-D-Glucuronide (BPA-G) on Adipogenesis in Human and Murine Preadipocytes.

ABSTRACT:

Background

Exposure to common environmental substances, such as bisphenol A (BPA), has been associated with a number of negative health outcomes. In vivo, BPA is rapidly converted to its predominant metabolite, BPA-glucuronide (BPA-G), which has long been believed to be biologically inactive because it lacks estrogenic activity. However, the effects of BPA-G on cellular metabolism have not been characterized. In the present study we examined the effect of BPA-G on adipogenesis.

Methods

The effect of BPA-G on the differentiation of human and 3T3L1 murine preadipocytes was evaluated in vitro by quantifying lipid accumulation and the expression of adipogenic markers.

Results

Treatment of 3T3L1 preadipocytes with 10 μM BPA-G induced a significant increase in lipid accumul

SUBMITTER: Boucher JG 

PROVIDER: S-EPMC4671229 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature