Rapid Identification of Emerging Human-Pathogenic Sporothrix Species with Rolling Circle Amplification.

ABSTRACT: Sporothrix infections are emerging as an important human and animal threat among otherwise healthy patients, especially in Brazil and China. Correct identification of sporotrichosis agents is beneficial for epidemiological surveillance, enabling implementation of adequate public-health policies and guiding antifungal therapy. In areas of limited resources where sporotrichosis is endemic, high-throughput detection methods that are specific and sensitive are preferred over phenotypic methods that usually result in misidentification of closely related Sporothrix species. We sought to establish rolling circle amplification (RCA) as a low-cost screening tool for species-specific identification of human-pathogenic Sporothrix. We developed six species-specific padlock probes targeting polymorphis

SUBMITTER: Rodrigues AM 

PROVIDER: S-EPMC4672047 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature