A functional connectome: regulation of Wnt/TCF-dependent transcription by pairs of pathway activators.

ABSTRACT:

Background

Wnt/β-catenin signaling is often portrayed as a simple pathway that is initiated by Wnt ligand at the cell surface leading, via linear series of interactions between 'core pathway' members, to the induction of nuclear transcription from genes flanked by β-catenin/TCF transcription factor binding sites. Wnt/β-catenin signaling is also regulated by a much larger set of 'non-core regulators'. However the relationship between 'non-core regulators' is currently not well understood. Aberrant activation of the pathway has been shown to drive tumorgenesis in a number of different tissues.

Methods

Mammalian cells engineered to have a partially-active level of Wnt/β-catenin signaling were screened by transfection for proteins that up or down-regulated a mid-level of TCF-dep

SUBMITTER: Freeman J 

PROVIDER: S-EPMC4672529 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature