A simple approach to evaluate the kinetic rate constant for ATP synthesis in resting human skeletal muscle at 7 T.

ABSTRACT: Inversion transfer (IT) is a well-established technique with multiple attractive features for analysis of kinetics. However, its application in measurement of ATP synthesis rate in vivo has lagged behind the more common saturation transfer (ST) techniques. One well-recognized issue with IT is the complexity of data analysis in comparison with much simpler analysis by ST. This complexity arises, in part, because the γ-ATP spin is involved in multiple chemical reactions and magnetization exchanges, whereas Pi is involved in a single reaction, Pi → γ-ATP. By considering the reactions involving γ-ATP only as a lumped constant, the rate constant for the reaction of physiological interest, kPi→γATP , can be determined. Here, we present a new IT data analysis method to evaluate kPi→γATP using dat

SUBMITTER: Ren J 

PROVIDER: S-EPMC4673044 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature