Generation of Kcnma1fl-tdTomato, a conditional deletion of the BK channel α subunit in mouse.

ABSTRACT: BK large conductance calcium-activated K(+) channels (KC a1.1) are expressed widely across many tissues, contributing to systemic regulation of cardiovascular, neurological, and other specialized physiological functions. The pore-forming α subunit is encoded by the Kcnma1 gene, originally named mSlo1 in mouse and slowpoke in Drosophila. Global deletion in mouse (Kcnma1(-/-)) produces a plethora of defects in neuron and muscle excitability, as well as other phenotypes related to channel function in nonexcitable cells. While homozygous null mice are viable, the ubiquitous loss of BK function has complicated the interpretation of phenotypes involving the interaction of multiple cell types which independently express BK channels. Here, we report the generation of a targeted allele for conditio

SUBMITTER: Zemen BG 

PROVIDER: S-EPMC4673641 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature