Design and expression of fusion protein consists of HBsAg and Polyepitope of HCV as an HCV potential vaccine.

ABSTRACT:

Background

Hepatitis C virus (HCV) infection is a serious public health threat worldwide. Cellular immune responses, especially cytotoxic T-lymphocytes (CTLs), play a critical role in immune response toward the HCV clearance. Since polytope vaccines have the ability to stimulate the cellular immunity, a recombinant fusion protein was developed in this study.

Materials and methods

The designed fusion protein is composed of hepatitis B surface antigen (HBsAg), as an immunocarrier, fused to an HCV polytope sequence. The polytope containing five immunogenic epitopes of HCV was designed to induce specific CTL responses. The construct was cloned into the pET-28a, and its expression was investigated in BL21 (DE3), BL21 pLysS, BL21 pLysE, and BL21 AI Escherichia coli strains using 1

SUBMITTER: Gholizadeh M 

PROVIDER: S-EPMC4673707 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature