The polarity protein Inturned links NPHP4 to Daam1 to control the subapical actin network in multiciliated cells.

ABSTRACT: Motile cilia polarization requires intracellular anchorage to the cytoskeleton; however, the molecular machinery that supports this process remains elusive. We report that Inturned plays a central role in coordinating the interaction between cilia-associated proteins and actin-nucleation factors. We observed that knockdown of nphp4 in multiciliated cells of the Xenopus laevis epidermis compromised ciliogenesis and directional fluid flow. Depletion of nphp4 disrupted the subapical actin layer. Comparison to the structural defects caused by inturned depletion revealed striking similarities. Furthermore, coimmunoprecipitation assays demonstrated that the two proteins interact with each other and that Inturned mediates the formation of ternary protein complexes between NPHP4 and DAAM1. Knockdo

SUBMITTER: Yasunaga T 

PROVIDER: S-EPMC4674276 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature