Up-regulation of microRNA-210 inhibits proliferation of hepatocellular carcinoma cells by targeting YES1.

ABSTRACT:

Aim

To determine the expression of microRNA-210 (miR-210) in hepatocellular carcinoma (HCC) and to examine its role using HCC cells.

Methods

The expression of miR-210 was determined in 21 pairs of HCC samples and the corresponding surrounding non-tumor tissues. The effects of miR-210 on proliferation and cell cycle progression were examined using HepG2 and HuH7 cells. Over-expression and inhibition of miR-210 was achieved by transfection of the cells with miR-210 mimic or inhibitor. Luciferase reporter constructs were used to identify the miR-210 interacting site on Yes1. Yes1 expression was examined after miR-210 transfection, as well as in the HCC samples.

Results

miR-210 was significantly up-regulated by 3.4 fold (P < 0.01) in the tumor samples. The over-expression

SUBMITTER: Tan W 

PROVIDER: S-EPMC4674721 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature